The Most Probable Number (MPN) Method – Let’s Expand our Toolbox in the QC Microbiology LabWebinar to be presented Thursday, May 31 at 2 pm ESTAbstractThe “Most Probable Number” (MPN) Method is a useful, if underutilized, tool for the microbiologist. It is part of the harmonized compendial chapter on bacterial enumeration and has been part of the Microbial Limits Test chapter in USP since the chapters inception in USP XVIII (1970). The test is a method to estimate the concentration of viable microorganisms in a sample by means of replicate liquid broth growth in ten-fold dilutions. It is particularly useful with samples that contain particulate material that interferes with plate count enumeration methods. The method offers real opportunities as a tool for microbiologists in situations where, for one reason or another, the plate count method is unsuitable. It can also be employed for semi-quantitative estimation of growth-promotion capability of liquid media, in estimation of precision for alternate microbiological methods, and as part of the “fraction negative” method of determining D-values. This webinar will review the MPN method and provide several suggestions of ways to use this method to expand the range of control that the QC lab can enjoy on media, microorganisms and on qualification of alternate (rapid) microbiological methods (RMM). Register Here!Scott Sutton, Ph. D.
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